Bead Mill Bacterial RNA Kit

Bead Mill Bacterial RNA Purification Kit - 50 Prep

Sku:

26-011B

Qty:

General Info

Bead Mill Bacteria RNA Purification Kit - 50 Prep

The Omni Bead Mill Bacteria RNA Purification Kit contains silica based spin-capture columns, pre-filled bead beating tubes and nontoxic reagents. The optimized reagents are designed specifically for RNA extraction from bacteria through bead mill homogenization and utilize highly denaturing buffer conditions to inactivate RNase's. After sample lysis using the Omni pre-filled bead tubes, the RNA is purified through spin-column capture in less than 60 minutes. RNA purified using the Omni Bacteria RNA Kit is ready for downstream applications such as RT-PCR. Pre-filled bead tubes are rigorously tested to be free of nucleases.

Product Features:

  • Complete RNA purification kit including pre-filled bead beating tubes, spin-columns and nontoxic reagents
  • RNA isolation in less than 60 minutes after lysis
  • No organic extractions
  • Suitable for up to 3 X 107 bacteria cells
  • Includes 2.0 mL polypropylene screw cap tubes pre-filled with 0.5 mm glass beads
  • Certification: Pre-filled bead tubes certified RNase & DNase Free

Illustrated Protocol

Bacterial RNA


Example Results

Total RNA purified using the Omni Bead Mill Bacterial RNA Purification Kit. RNA was extracted from Esherichia coli (E. coli) at 4.4 x 10^8, 2.2 x 10^8, and 1.1 x 10^8 cfu/mL on the Bead Ruptor Elite, in pre-filled 2 mL tubes with 0.5 mm glass beads at 3.1 m/s for 60 sec. Purified RNA was analyzed on the Agilent 2100 Bioanalyzer. Average RNA Integrity Numbers (RIN) was 8.5.



Ordering Information: Unit includes 50 spin-columns, 50 bead beating tubes prefilled with 2.8 mm ceramic beads, reagents suitable for 50 purifications and instruction guide

Specifications

  • RNA purity: High quality genomic DNA with A260/A280 > 1.8
  • RNA yield: Up to 100 ug
  • Capacity: Capable of RNA extraction from up to 3 X 107 bacteria cells
  • Bead tube material: Polypropylene
  • Bead tube volume: 2.0 mL
  • Bead material: 0.5 mm glass
  • Equipment required: Microcentrifuge, RNase Free 1.5 mL microcentrifuge tubes, water bath, heat block, 100% ethanol