Omni's Application Database header image

Mosquito DNA Extraction and High Throughput Gene Targeting on Bead Ruptor 96 Bead Mill Homogenizer

Authors: C Proctor, S Soldat, B Easparro, R Nash, J Atwood

Animal

Application Info

Description:

Mosquitos, specifically Aedes aegypti, are known to act as a host for vector borne diseases such as Yellow fever, Dengue fever, and Zika (1). Most of these diseases are associated with tropical climates, but Aedes aegypti are found throughout the Southeast United States, with a likely range from Virginia to Texas (2). To monitor the spread of vector borne diseases, researchers typically sample mosquitos from the field and dissociate the organism to release the internal pathogens to quantify pathogen load. Researchers require a fast and repeatable sample preparation technique to rapidly dissociate a large number of mosquitos while maintaining a high degree of vector and pathogen lysis. Herein, we demonstrate dissociation of Aedes aegypti using the Bead Ruptor 96 bead mill homogenizer for the purification of DNA and analysis by endpoint PCR.

Goal:

DNA was extracted from mosquitos to be used for PCR using the Bead Ruptor 96.

Mosquito DNA Extraction and High Throughput Gene Targeting on Bead Ruptor 96 Bead Mill Homogenizer

Sample Type:

Animal

Tags:

  • Bead Ruptor 96
  • mosquitos
  • DNA
  • PCR
  • gene targeting

Created On:

2018-12-13

×

To download this PDF, you must be logged into your Omni account.