Mosquitos, specifically Aedes aegypti, are known to act as a host for vector borne diseases such as Yellow fever, Dengue
fever, and Zika (1). Most of these diseases are associated with tropical climates, but Aedes aegypti are found throughout
the Southeast United States, with a likely range from Virginia to Texas (2). To monitor the spread of vector borne diseases,
researchers typically sample mosquitos from the field and dissociate the organism to release the internal pathogens to
quantify pathogen load. Researchers require a fast and repeatable sample preparation technique to rapidly dissociate a large
number of mosquitos while maintaining a high degree of vector and pathogen lysis. Herein, we demonstrate dissociation
of Aedes aegypti using the Bead Ruptor 96 bead mill homogenizer for the purification of DNA and analysis by endpoint PCR.